Metabolomics is the study of global small-molecule (<1500 Da) profiles in a system (cell, tissue, or organism). Together with genomics, transcriptomics, proteomics and other -omics sciences, it contributes to the understanding of global systems biology.

The main analytical tools in metabolomics are mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, each having different strengths and weaknesses. Metabolomic studies rely on two main strategies: i) targeted metabolomics, for hypothesis-driven experiments, that focus on a few specific metabolic pathways/sets of biomarkers expected to play a role in the biochemical problem under examination; ii) untargeted metabolomics, to measure ideally the entire set of small molecules in  a biological sample providing a global picture of the metabolome.